1817 mRNA fragments for dsRNA creation from third batch, May 30, 2011.

1. 100% agreement with gene from Official Gene Set (OGS) or from AUGUSTUS prediction ("revised", including UTRs) using cDNA hints
2. minimum length of 500bp
3. 100% cDNA coverage (at least 5-fold at each position)
4. unspliced AUGUSTUS genes were only trusted if they had a significant BLAST hit (E < 1e-5)
5. at least 6kbp apart from all other mRNA fragments and former dsRNA-templates or separated from closer ones
   
   • by an EST terminus or
   • different strandedness
6. not aligning to the same transcript as another mRNA fragment or at least 50kbp apart in the genome