3498 "safe" mRNA fragments for dsRNA creation from second batch, Nov 29, 2010.

"Safe" mRNA fragments satisfy:

1. minimum length of 500bp
2. has 100% cDNA coverage (at least 5-fold at each position)
3. agrees 100% with gene from Official Gene Set (OGS) or from AUGUSTUS ab initio prediction
4. at least 3Kb apart from all other "safe" mRNA fragments
or separated by an EST terminus from closer mRNA fragments
5. does not align to the same transcript as another safe mRNA fragment from second or first batch
6. unspliced ab initio predicted AUGUSTUS genes were not trusted because of the possibility of getting transposed elements

Contact: Mario Stanke

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