1384 mRNA fragments for dsRNA creation from fourth batch, January 7, 2014.

1. 100% agreement with au3 transcripts (reannotation of T. castaneum Assembly 4.0)
2. minimum length of 500bp
3. 100% cDNA coverage (at least 5-fold at each position)
4. unspliced au3 genes were only trusted if they had a significant BLAST hit (E < 1e-5)
5. at least 4kbp apart from all other mRNA fragments and former dsRNA-templates or separated from closer ones
   
   • by an EST terminus or
   • different strandedness
6. not aligning to the same transcript as another mRNA fragment
7. each fragment satisfies at least one of the following criteria
   
   • is homolog to a D. melanogaster protein of unknown function
   • is highly conserved in Drosophila and others (at least 50 hits in uniprot-sprot-invertebrates database)
   • is ortholog in other species but not in Drosophila
   • is associated to one of the following GO-terms from molecular function onthology
     
     • GO:0060089 : molecular transducer activity
       
       • GO:0004871 : signal transducer activity
         
         • GO:0009927 : histidine phosphotransfer kinase activity
         • GO:0000156 : phosphorelay response regulator activity
         • GO:0005057 : receptor signaling protein activity
         • GO:0038023 : signaling receptor activity
     • GO:0016015 : morphogen activity, or its child terms
     • GO:0001071 : nucleic acid binding transcription factor activity, or its child terms
     • GO:0000988 : protein binding transcription factor activity, or its child terms
     • GO:0045182 : translation regulator activity
       
       • GO:0045183 : translation factor activity, non-nucleic acid binding
       • GO:0090079 : translation regulator activity, nucleic acid binding
         
         • GO:0008494 : translation activator activity
       • GO:0030371 : translation repressor activity
         
         • GO:0000900 : translation repressor activity, nucleic acid binding
         • GO:0000901 : translation repressor activity, non-nucleic acid binding
     • GO:0004872 : receptor activity
       
       • GO:0038023 : signaling receptor activity
       • GO:0038187 : pattern recognition receptor activity
         
         • GO:0008329 : signaling pattern recognition receptor activity
     • GO:0030545 : receptor regulator activity
       
       • GO:0030546 : receptor activator activity
         
         • GO:0030297 : transmembrane receptor protein tyrosine kinase activator activity
       • GO:0030547 : receptor inhibitor activity
         
         • GO:0030293 : transmembrane receptor protein tyrosine kinase inhibitor activity
     • GO:0032502 : developmental process (biological process)