Prediction wit RNA-seq in the form of PE SOLiD reads
Posted: Thu Nov 19, 2015 3:37 pm
Originally posted in the old forum by boewig on 27.06.2013 - 16:59
I have Paired reads from SOLiD (50bp + 35bp) which i would like to use in order to make better gene calling of a filamentous fungi.
However when mapping with SHRIMP I'm unsure whether i should use the paired information in the alignment or not. Do you have any experience with this?
/Kasper
I have Paired reads from SOLiD (50bp + 35bp) which i would like to use in order to make better gene calling of a filamentous fungi.
However when mapping with SHRIMP I'm unsure whether i should use the paired information in the alignment or not. Do you have any experience with this?
/Kasper