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Prediction wit RNA-seq in the form of PE SOLiD reads

Posted: Thu Nov 19, 2015 3:37 pm
by katharina
Originally posted in the old forum by boewig on 27.06.2013 - 16:59
I have Paired reads from SOLiD (50bp + 35bp) which i would like to use in order to make better gene calling of a filamentous fungi.
However when mapping with SHRIMP I'm unsure whether i should use the paired information in the alignment or not. Do you have any experience with this?
/Kasper

Re: Prediction wit RNA-seq in the form of PE SOLiD reads

Posted: Thu Nov 19, 2015 3:37 pm
by katharina
by katharina on 13.08.2014 - 11:33
I would always use the pairedness information if available.
Katharina