bam2hints created an empty gff file
I am trying ab-initio gene prediction on my species.
According to this page (http://bioinf.uni-greifswald.de/bioinf/ ... seq.Tophat), I attempted to get intron hints from several sets of RNAseq data, but at step 11, bam2hints created an empty .gff file.
I think it's not error because there was no on terminal and it just said like:
Wait a moment, calculating maximum block size that needs to be allocated... .. done
I suspected I happened to make some mistakes at previous steps, but I could not figure out.