RNASeq data for training and hintsfile
Posted: Thu Nov 19, 2015 5:21 pm
Originally posted in the old forum by chrikus on 20.03.2015 - 09:18
hey,
I have several sets of RNAseq data and map it with segemehl.
I´m trying to follow the manuals of incorparating RNAseq, but I don´t know, how to solve the problem with the several data sets.
1) Can I use the merged sets as input for augustus?
or is it better to merge it afterwards?
2) How can I prepare RNAseq data for training?
different approach:
is it possible to create hintsfile with PASA output? or PASA can only be used for training?
thanks in advanced,
chrikus
hey,
I have several sets of RNAseq data and map it with segemehl.
I´m trying to follow the manuals of incorparating RNAseq, but I don´t know, how to solve the problem with the several data sets.
1) Can I use the merged sets as input for augustus?
or is it better to merge it afterwards?
2) How can I prepare RNAseq data for training?
different approach:
is it possible to create hintsfile with PASA output? or PASA can only be used for training?
thanks in advanced,
chrikus