Hello,
I am trying to use augustus to annotate our genome [...]
Apparently you already have trained augustus with Tetrahymena, which is the closest model organism.
I have ESTs and I have generated a hint file :
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scaffold51_5 EST51 exonpart 127840 127889 116.6 + . grp=ESTPt5100000000379;pri=4;src=E
scaffold51_5 EST51 intron 127890 127915 116.6 + . grp=ESTPt5100000000379;pri=4;src=E
scaffold51_5 EST51 exon 127916 128173 116.6 + . grp=ESTPt5100000000379;pri=4;src=E
scaffold51_5 EST51 intron 128174 128199 116.6 + . grp=ESTPt5100000000379;pri=4;src=E
scaffold51_5 EST51 exon 128200 128535 116.6 + . grp=ESTPt5100000000379;pri=4;src=E
scaffold51_5 EST51 intron 128536 128564 116.6 + . grp=ESTPt5100000000379;pri=4;src=E
scaffold51_5 EST51 exonpart 128565 128736 116.6 + . grp=ESTPt5100000000379;pri=4;src=E
scaffold51_122 EST51 exonpart 109994 110207 117.3 - . grp=ESTPt5100000027354;pri=4;src=E
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augustus --species=[...] $REF --UTR=off --strand=both --genemodel=complete --AUGUSTUS_CONFIG_PATH=$AUGUSTUS_CONF --alternatives-from-evidence=false --hintsfile=hints.gff
Error: intron hint is too short.
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scaffold51_2 EST51 intron 11430 11457 230.7 + . grp=ESTPt5100000046600;pri=4;src=E "100436;0.34;0:0"
Delete group HintGroup ESTPt5100000046600, 10920-11643, mult= 1, priority= 4 3 features
Error: intron hint is too short.
Could you help me ?